Is HappyFect™ transfection reagent difficult to use?
No, all of our HappyFect™ transfection reagent products are easy to use involving only a few simple steps. We understand how important it is for you to get on with your experiments. Our simple protocols also help to improve reproducibility.
Do I need any special equipment to use HappyFect™?
No, all of our HappyFect™ protocols involve only standard lab equipment. Happily, there is no need to purchase new equipment or specialised disposables.
Is HappyFect™ toxic to cells in any kind of way?
No. HappyFect™ products are effectively non-toxic and thus it is particularly recommended for transfection of highly-sensitive cells. Of course, all substances are toxic at some level, but we are confident that HappyFect™ products are remarkably non-toxic and particularly so relative to other transfection technologies. We take pride in working to ensure that HappyFect does not make your cells unhappy. Relative toxicity data can be viewed within our “Examples” page and we can provide you with further toxicity data if of interest.
Are optimal transfection conditions cell line-dependent?
Yes, each cell line may require optimisation of transfection conditions. The HappyFect™ protocols provide a convenient starting point. At Tecrea Ltd we are constantly working to provide optimum protcols for specific cell lines eg CHO, HEK, HELA, and many more. The results will be published on our website and you are always welcome to contact our scientists for advice and suggestions.
Is cell density important for consideration?
Yes, cell density will affect transfection efficiency. We recommend using HappyFect™ products at 50-70% confluence.
Can antibiotics be used in media during transfection?
HappyFect™ products work well in the presence or absence of antibiotics properties.
Can I perform transfections in the presence of serum?
Yes, HappyFect™ products work in the presence of serum. However, as with most or all transfection reagents, HappyFect™ is more efficient when used in the absence of serum. Therefore, to optimise results, particularly when working with difficult to transfect cells, we suggest transfection in the absence of serum.
How critical is the length of transfection complex formation incubation period?
The complexation time is not critical, but we recommend using a consistent incubation period when conducting different experiments.
Is it possible to perform multiple transfections on cells?
Yes. It is well established that HappyFect™ products are effectively non-toxic (see data within examples). Therefore, it is possible to perform multiple consecutive transfections on cells in order to obtain a prolonged effect of overexpression or knockdown.
What types of molecules can be transfected using happyFect?
HappyFect™ products can be used to transfect DNA, siRNA, antisense DNA, PNA, oligonucleotides, and proteins including antibodies and peptides.
Can HappyFect™ products be used to co-transfect more than one plasmid simultaneously?
Yes, HappyFect™ products have been validated to effectively and reproducibly co-transfect DNA plasmids to variety of mammalian cells.
What is the stability of HappyFect™?
HappyFect™ products are very stable. It can be shipped at room temperature and can be stored at -4oC for at least 2 years. HappyFect™ products should be protected from light. Indeed, for up to one month it is fine to store HappyFect™ products at room temperature on the bench.
What is nanotechnology?
HappyFect™ works by forming nanoparticles with nucleic acids – but what are nanoparticles and what is nanotechnology? Rather than give you a long winded explaination, we will simply point you to sources that offer useful insight:
http://www.nano.org.uk/what-is-nanotechnology
http://en.wikipedia.org/wiki/Nanotechnology
If your question is not answered above, please contact
info@tecrea.co.uk
